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Name

Dr. Valentine Otang Ntui

Department

Genetics and Biotechnology

Designation

Senior Lecturer

Email

[email protected]

About Dr. Valentine Otang Ntui

Dr Valentine Otang Ntui is a citizen of Nigeria, a scientist at International Institute of Tropical Agriculture (IITA), Nairobi station, Kenya, and teacher at the Department of Genetics and Biotechnology, University of Calabar, Nigeria. He joined the services of the University in 2001 as Graduate Assistant and was promoted Senior Lecturer in 2015, teaching courses in Plant Biotechnology, Molecular Biology and genome editing, also researching in the field of Genetic Engineering and Genome Editing. He has attended a couple of local and international conferences. In 2010, he obtained Ph.D. degree in Plant Biotechnology (Plant Genetic Engineering) from Chiba University, Japan. From 2014 to 2017, he was a postdoctoral fellowship at the Centre for Desert Agriculture, King Abdullah University of Science and Technology (KAUST), Jeddah, Saudi Arabia. Prior to joining KAUST, he was a Japan Society for the Promotion of Science (JSPS) postdoctoral fellow at the Laboratory of Plant Cell Technology, Chiba University (Japan), where his main area of research was on the engineering of a variety of crop plants (cassava, potato, tomato, tobacco) for resistance to viruses using RNAi Technology. His research interests include molecular biology, plant-tissue culture and genetic transformation, in vitro development of polyploids and germplasm conservation. His current focus of research is on CRISPR/Cas9 mediated genome editing in plants for resistance to biotic/abiotic stresses. His notable awards include Japan Society for the Promotion of Science postdoctoral fellowship and Monbukagakusho (Government of Japan, MEXT) Ph.D scholarship. He is a member of International Association for Plant Biology and Society for In vitro Biology. Ntui has a total of 50 publications in international journals.

S/N Qualification School Atttended Started Finished
GCE O;LevelGovernment Secondary School, Eyumjock, Cameroun19831988
GCE A'LevelGovernment High School, Mamfe, Cameroun19881990
B.Sc. BiologyUniversity Of Calabar, Calabar., Cross River State, Nigeria19931997
M.Sc. Genetics and Plant breedingUniversity Of Calabar, Calabar., Cross River State, Nigeria20002003
PhD Plant BiotechnologyChiba University, Japan20072010
S/N Publication
Tripathi, L., Ntui, V. O., & Tripathi, J. N. (2019). Application of genetic modification and genome editing for developing climate‐smart banana. Food and Energy Security, e00168.
Haider, I., Andreo-Jimenez, B., Bruno, M., Bimbo, A., Floková, K., Abuauf, H., ... & Bouwmeester, H. J. (2018). The interaction of strigolactones with abscisic acid during the drought response in rice. Journal of experimental botany, 69(9), 2403-2414.
Haider, I., Andreo-Jimenez, B., Bruno, M., Bimbo, A., Floková, K., Abuauf, H., ... & Bouwmeester, H. J. (2018). The interaction of strigolactones with abscisic acid during the drought response in rice. Journal of experimental botany, 69(9), 2403-2414.
Kim, D., Abdelaziz, M. E., Ntui, V. O., Guo, X., & Al-Babili, S. (2017). Colonization by the endophyte Piriformospora indica leads to early flowering in Arabidopsis thaliana likely by triggering gibberellin biosynthesis. Biochemical and biophysical research communications, 490(4), 1162-1167.
Ntui, V. O., Uyoh, E. A., Nakamura, I., & Mii, M. (2017). Agrobacterium-mediated genetic transformation of Fonio (Digitaria exilis (L.) Stapf). African Journal of Biotechnology, 16(23), 1302-1307.
Kim, D., Ntui, V. O., & Xiong, L. (2016). Arabidopsis YAK 1 regulates abscisic acid response and drought resistance. FEBS letters, 590(14), 2201-2209.
Ntui, V. O., Kong, K., Khan, R. S., Igawa, T., Janavi, G. J., Rabindran, R., ... & Mii, M. (2015). Resistance to Sri Lankan cassava mosaic virus (SLCMV) in genetically engineered cassava cv. KU50 through RNA silencing. PLoS One, 10(4), e0120551.
Kim, D., Ntui, V. O., Zhang, N., & Xiong, L. (2015). Arabidopsis Yak1 protein (AtYak1) is a dual specificity protein kinase. FEBS letters, 589(21), 3321-3327.
Phlaetita, W., Chin, D. P., Otang, N. V., Nakamura, I., & Mii, M. (2015). High efficiency Agrobacterium-mediated transformation of Dendrobium orchid using protocorms as a target material. Plant Biotechnology, 15-0804.
Kong, K., Ntui, V. O., Makabe, S., Khan, R. S., Mii, M., & Nakamura, I. (2014). Transgenic tobacco and tomato plants expressing Wasabi defensin genes driven by root-specific LjNRT2 and AtNRT2. 1 promoters confer resistance against Fusarium oxysporum. Plant Biotechnology, 13-1209.
Darwish, N. A., Khan, R. S., Ntui, V. O., Nakamura, I., & Mii, M. (2014). Generation of selectable marker-free transgenic eggplant resistant to Alternaria solani using the R/RS site-specific recombination system. Plant cell reports, 33(3), 411-421.
Ntui, V. O., Kynet, K., Khan, R. S., Ohara, M., Goto, Y., Watanabe, M., ... & Mii, M. (2014). Transgenic tobacco lines expressing defective CMV replicase-derived dsRNA are resistant to CMV-O and CMV-Y. Molecular biotechnology, 56(1), 50-63.
Kong, K., Makabe, S., Ntui, V. O., Khan, R. S., & Nakamura, I. (2014). Synthetic chitinase gene driven by root-specific LjNRT2 and AtNRT2. 1 promoters confers resistance to Fusarium oxysporum in transgenic tobacco and tomato. Plant biotechnology reports, 8(2), 151-159.
Khan, R. S., Darwish, N. A., Khattak, B., Ntui, V. O., Kong, K., Shimomae, K., ... & Mii, M. (2014). Retransformation of marker-free potato for enhanced resistance against fungal pathogens by pyramiding chitinase and wasabi defensin genes. Molecular biotechnology, 56(9), 814-823.
Ntui, V. O., Kong, K., Azadi, P., Khan, R. S., Chin, D. P., Igawa, T., ... & Nakamura, I. (2014). RNAi-mediated resistance to cucumber mosaic virus (CMV) in genetically engineered tomato. American Journal of Plant Sciences, 5(05), 554.
Ntui, V. O., Kong, K., Azadi, P., Khan, R. S., Chin, D. P., Igawa, T., ... & Nakamura, I. (2014). RNAi-mediated resistance to cucumber mosaic virus (CMV) in genetically engineered tomato. American Journal of Plant Sciences, 5(05), 554.
Sharafi, A., Sohi, H. H., Mousavi, A., Azadi, P., Razavi, K., & Ntui, V. O. (2013). A reliable and efficient protocol for inducing hairy roots in Papaver bracteatum. Plant Cell, Tissue and Organ Culture (PCTOC), 113(1), 1-9.
Ntui, V. O., Kynet, K., Azadi, P., Khan, R. S., Chin, D. P., Nakamura, I., & Mii, M. (2013). Transgenic accumulation of a defective cucumber mosaic virus (CMV) replicase derived double stranded RNA modulates plant defence against CMV strains O and Y in potato. Transgenic research, 22(6), 1191-1205.
Urua, I. S., Uyoh, E. A., Ntui, V. O., & Okpako, E. C. (2013). Effect of processing on proximate composition, anti-nutrient status and amino acid content in three accessions of African locust bean (Parkia biglobosa (jacq.) benth. International journal of food sciences and nutrition, 64(1), 94-102.
Mirmasoumi, M., Azadi, P., Sharafi, A., Ntui Otang, V., & Mii, M. (2013). Simple protocol for plant regeneration of Lilium ledebourii using transverse thin cell layer. Progress in Biological Sciences, 3(2), 117-122.
Azadi, P., Ntui, V. O., Chin, D. P., & Mii, M. (2013). Genetic transformation and metabolic engineering of Lilium. Bulbous Plants: Biotechnology, 197.
Udensi, O., & Ntui, V. O. (2013). Evaluating the Performance of Amiprophos Methyl and [gamma]-irradiated Seeds on Growth and Yield Traits of Pigeon Pea. International Journal of Agriculture and Biology, 15(5).
Udensi, O., & Ntui, V. O. (2013). Evaluating the Performance of Amiprophos Methyl and [gamma]-irradiated Seeds on Growth and Yield Traits of Pigeon Pea. International Journal of Agriculture and Biology, 15(5).
Ntui, V. O., Kynet, K., Nakamura, I., & Mii, M. (2013, June). RNAi-mediated Resistance to Cucumber Mosaic Virus (CMV) in Transgenic Tomato. In IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL (Vol. 49, pp. S37-S38). 233 SPRING ST, NEW YORK, NY 10013 USA: SPRINGER.
Ntui, V. O., Uyoh, E. A., Urua, I. S., Ogbu, U., & Okpako, E. C. (2012). Regeneration of Parkia biglobosa Benth.: an important tree species of Africa. J Microbiol Biotechnol Res, 2(1), 169-177.
Khan, R. S., Ntui, V. O., Chin, D. P., Nakamura, I., & Mii, M. (2011). Production of marker-free disease-resistant potato using isopentenyl transferase gene as a positive selection marker. Plant cell reports, 30(4), 587-597.
Uyoh, E. A., Urua, I. S., Ntui, V. O., & Okpako, E. C. (2011). Flow cytometric analysis of nuclear DNA content, mitotic chromosome number and protein separation by SDS-PAGE in three accessions of African locust bean (Parkia biglobosa Benth.). Journal of Crop Science and Biotechnology, 14(4), 227-232.
Ntui, V. O., Azadi, P., Thirukkumaran, G., Khan, R. S., Chin, D. P., Nakamura, I., & Mii, M. (2011). Increased resistance to fusarium wilt in transgenic tobacco lines co‐expressing chitinase and wasabi defensin genes. Plant pathology, 60(2), 221-231.
Ntui, V. O., Thirukkumaran, G., Azadi, P., Khan, R. S., Nakamura, I., & Mii, M. (2010). Stable integration and expression of wasabi defensin gene in “Egusi” melon (Colocynthis citrullus L.) confers resistance to Fusarium wilt and Alternaria leaf spot. Plant cell reports, 29(9), 943-954.
Ntui, V. O., Chin, D. P., Nakamura, I., & Mii, M. (2010). An efficient Agrobacterium tumefaciens-mediated genetic transformation of “Egusi” melon (Colocynthis citrullus L.). Plant Cell, Tissue and Organ Culture (PCTOC), 103(1), 15-22.
Thirukkumarana, G., Ntui, V. O., Khan, R. S., Nakamura, I., & Mii, M. (2010). Generation of phenotypically normal marker-free transgenic plants of Kalanchoe blossfeldiana through hairy root induction. Plant biotechnology, 27(2), 147-153.
Ntui, V. O., Thirukkumaran, G., Iioka, S., & Mii, M. (2009). Efficient plant regeneration via organogenesis in “Egusi” melon (Colocynthis citrullus L.). Scientia Horticulturae, 119(4), 397-402.
Thirukkumaran, G., Ntui, V. O., Khan, R. S., & Mii, M. (2009). Thidiazuron: an efficient plant growth regulator for enhancing Agrobacterium-mediated transformation in Petunia hybrida. Plant Cell, Tissue and Organ Culture (PCTOC), 99(1), 109-115.
Koornneef, M., Reuling, G., & Karssen, C. M. (1984). The isolation and characterization of abscisic acid‐insensitive mutants of Arabidopsis thaliana. Physiologia Plantarum, 61(3), 377-383.
S/N Grant Currency/Amount Grantor Date
Research Grant SecuredUSD 500000Bill and Melinda Gates foundation.2015-10-01
Engineering yam for resistance to anthracnose by RNA silencingNGN 33000000TETFUND2019-06-15
S/N Conference Description Started Finished
Annual conference of Agricultural Society of Nigeria, Calabar, Nigeria.Ntui VO, Uyoh EA (2003) Intraspecific hybridization in ‘egusi’ melon, Colocynthis citrullus L. 2003-10-012003-10-01
9th IPMB congress, St. Louis, Missouri, USA.Ntui VO, Khan RS, Nakamura I, Mii M (2009) Agrobacterium-mediated genetic transformation of “Egusi” melon (Colocynthis citrullus L.) for resistance to Fusarium wilt and Alternaria leaf spot. 2009-10-252009-10-30
In vitro Biology Meeting and IAPB 12th world congress. St. Louis, Missouri, USA.Ntui VO, Azadi P, Gunaratnam T, Khan RS, Nakamura I, Mii M (2010). Increased resistance to Fusarium wilt in transgenic tobacco lines co-expressing chitinase and wasabi defensin genes .2010-06-062010-06-11
The 5th International Symposium Breeding Research on Medicinal and Aromatic Plants, Vienna, AustriaUyoh EA, Ntui VO, Osuagwu AN, Okpako EC, Ene-Obong EE, Ekpo I, Urua IS, Otu P (2012) Domestication and characterisation of some endangered indigenous spice plants in Nigeria. 2012-06-182012-06-20
Plant Biology Congress, Freiburg, GermanyNtui VO, Kynet K, Khan RS, Chin DP, Nakamura I, Mii M (2012). Production of transgenic potato plants for resistance to CMV by RNA silencing. 2012-07-292012-08-03
. In vitro Biology Meeting, Providence, Rhode Island, USA.Ntui VO, Kynet K, Nakamura I, Mii M (2013). RNAi-mediated resistance to cucumber mosaic virus in transgenic tomato2013-06-142013-06-19